Next Generation Sequencing with Illumina Genome Analyzer and its Applications	a guarantee of excellence
	New: Indexing & Targeted Sequencing now available!

Description

As a leading provider of genomic research service in the UK, Geneservice is pleased to offer the Illumina Genome Analyzer technology.

Genome Analyzer Technology

Based on revolutionary Solexa® Sequencing technology, the Illumina Genome Analysis system is a groundbreaking new platform for genetic analysis and functional genomics. It dramatically improves speed and reduces costs. Leveraging proprietary reversible terminators and Clonal Single Molecule Array technology, the Illumina Genome Analysis system has the potential to generate upwards of one to three billion bases of data per run, and in the process transform the way many experiments are devised and carried out.

Applications of Genome Analyzer

1. Genome Sequencing and Resequencing
   • Discover and confirm SNPs
   • Identify chromosomal rearrangements, including Copy Number Variations (CNVs)
   • Map break points
   • Detect rare variants
   
   
2. Transcriptome Analysis

   Generates genome-wide expression profiles through sequencing and not hybridization.
   Illumina offers four Transcriptome Analysis applications:

   • mRNA Sequencing (mRNA-seq)
     • mRNA-Seq allows profiling the whole population of mRNA in any eukaryotic species and enables mapping and digital quantification of whole transcripts. It also provides a rich source of sequence data for assessing alternative splice events.
   • Small RNA Discovery and Analysis
     • Discovery and profiling of all forms of small non-coding RNA including miRNA analysis
     • Unbiased discovery platform - no prior sequence or secondary structure information required
   • DeepCAGE Analysis
     • Cap-Analysis-Gene-Expression in combination with Next Generation Sequencing offers unmatched genome-wide views on promoter activities and transcripts.
     • Useful for promoter identification, transcriptional network analysis and transcriptome characterisation.
   
   
3. ChIP-Seq Application

   ChIP-Seq combines chromatin immunoprecipitation (ChIP) with Solexa Sequencing technology to identify and quantify in vivo protein-DNA interactions on a genome-wide scale. Sequencing ChIP-enriched DNA fragments enables identification of binding sites of DNA-binding proteins to precisely and cost effectively map all binding sites for a protein of interest to any sequenced genome.

4. DNA Methylation

   Discover methylation variable positions across the whole genome by sequencing from:

   • Methylation sensitive restriction digests
   • Bisulfite conversion
   • Anti-methyl C precipitation

Expanded Applications

1. Sample Indexing

   DNA samples are tagged with a unique identifier during sample preparation and up to 12 samples can be pooled into a single lane to be sequenced together, saving time and a cost effective solution for sequencing.

2. Targeted Sequencing

   Specific targeted genomic regions (either contiguous or dispersed) are enriched for subsequent deep sequencing. The ability to analyse only targeted regions rather than the entire complex genome allows for effective and systematic in-depth study of genetic variation, enabling researchers to work with larger number of samples. Geneservice is pleased to offer target-specific sequence selection through Agilent SureSelect™ and NimbleGen Sequence Capture.

Bioinformatics

Our Bioinformatics scientists provide a complete analysis package that can also be customised to your specific research needs.

Certifications:

We are a CSPro certified provider of Illumina sequencing.

Wilson NK, Miranda-Saavedra D, Kinston S, Bonadies N, Foster SD, Calero-Nieto F, Dawson MA, Donaldson IJ, Dumon S, Frampton J, Janky R, Sun XH, Teichmann SA, Bannister AJ, Gottgens B, The transcriptional programme controlled by the stem cell leukaemia gene Scl/Tal1 during early embryonic haematopoietic development, Blood, 3 April 2009. DOI 10.1182/blood-2009-01-200048.

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