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Large Construct Sequencing Service

Full sequencing of your BAC, YAC or Cosmid clones by Shotgun Sequencing or Next Generation Sequencing is now available at Geneservice.

Full sequencing of large constructs such as BAC, YAC or Cosmid clones/libraries can be challenging and time consuming using Sanger sequencing. Next Generation Sequencing using the Illumina Genome Analyzer II, has dramatically improved speed and reduced costs, enabling the full sequencing of large constructs at a fraction of the costs and time.

Take advantage of our introductory offer and have your BAC, YAC or Cosmid clones sequenced for £999 !

Sample Requirements:
  • 1µg of extracted DNA
Service Specifications:
  • Library Preparation
  • Cluster generation
  • Sequencing by synthesis (38bp reads)
  • Report generation and data delivery
Deliverables:
  • Approximately 10Mb sequence data obtained per construct. (Equivalent to 50x coverage for a 200kb BAC).
  • Assembly of sequence data by alignment against the relevant reference genome sequence.

Our bioinformaticians can help you if you require advanced sequence analysis (eg: de novo assembly).

Contact:
Email : Sequencing Team

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