Whole Genome Amplification
WGA has been shown to work successfully on a number of different starting materials including;
Whole Blood, Blood Cards, Plasma/Serum Samples, Fresh/frozen tissues (normal/tumor), Urine, Buccal Swabs,
Buffy Coat, Mouthwash.
The preparation of genomic DNA from clinical samples is often a bottleneck in genotyping and DNA sequencing
analysis and is frequently limited by the amount of specimen available. Whole Genome Amplification by
Geneservice is ideal for amplifying good-quality genomic DNA from small amounts of whole blood, dried
blood, buccal cells, cultured cells, and buffy coats specimens, fresh/frozen tissue generating large
amounts of DNA for all downstream applications.
Whole Blood is the most common source of material when performing genetic studies. In cases where only a
small volume of blood is available, WGA can be performed directly on the blood. This eliminates the need
to extract DNA and may be a cost effective solution for obtaining large quantities of DNA.
Buccal Swabs often result in low quantity and low quality genomic DNA which can hinder downstream
applications. WGA can be used to immortalize DNA for many down stream applications.
Blood Cards are an easy and convenient way to archive small volumes of blood, but unfortunately often
yield very small amounts of genomic DNA, which is low quality and unusable for downstream applications.
WGA allows the amplification of genomic DNA to microgram amounts for any number for downstream applications,
with minimal amplification bias.
Plasma/Serum Samples If archived biological specimens were collected prior to routine genotyping, serum or plasma is often the
only source of DNA. WGA on plasma/serum samples will work if there are enough cells or free DNA present in
the sample. WGA by Geneservice allows the quick and reproducible amplification of genomic DNA from plasma
samples, and is ideal for all downstream genotyping applications.
Laser Capture Microdissected Tissue The combination of laser capture microdissection, whole-genome amplification, and microarray or genotyping
techniques provides a powerful combination in advancing disease progression. WGA has been used to
successfully amplify genomic DNA from LCM tissue.
Plant Tissue Extracting DNA from plant tissue is a complicated procedure due to the tough cell wall that surrounds most
plant cells. Genomic DNA from plants is often poor quality and of low yield. WGA offered by Geneservice
provides an ideal solution to amplifying nanogram amounts of genomic DNA from plant material with little or
no detectable bias.
Soil Samples Extracting and amplifying genomic DNA from soil samples is a complicated procedure as the DNA is often
damaged or of low yield. DNA extraction and WGA by Geneservice provides a quick and cost effective route
to rescue such samples.
Sorted Chromosomes Whole Genome Amplification is particularly efficient at amplifying single copies of chromosomes for the
production of chromosome paints.
Other sample types Cultured Cells, Single Cells, Microbial Cells, Purified Genomic DNA from any source
If your sample type is not on our list, it does not necessarily mean we are unable to process your sample.
Please contact us to discuss your project in more detail.
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