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Approved research providers of
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Applied Biosystems SNPlex ™ 48-plex SNP genotyping system.
Description
Single Nucleotide Polymorphisms are bi-allelic, single base-pair changes between the DNA
sequences of an individual. They can exist within a population at any frequency although those
with a frequency above 20% are generally most useful in genetic mapping studies. SNPs are found
throughout the genome averaging one SNP every 500 to 1000bp. They can affect translation and
transcription. In some circumstances they lead to profound disease whereas elsewhere will have
no obvious effect. SNPs have also been used in pharmacogenomic experiments to predict why some
individuals respond better to certain therapeutics.
The SNPlex ™ Genotyping System uses an Applied Biosystems oligonucleotide ligation assay
(OLA) combined with multiplex PCR technology to achieve allelic discrimination and target
amplification.
Geneservice are an approved service provider for Applied Biosystems SNPlex system.
Application
The special virtues of SNPs for genetic analysis are their ease of discovery and assay, and
their ease of analysis. Their high density in the human genome and their effects, in some
cases, on transcription and translation make SNPs valuable for association studies. The
SNPlex platform is ideal for use in screening candidate genes for associations or for
confirming genome-wide associations in follow-up populations.
SNPlex studies are of the order of 100s of SNPs genotyped in 1000s of DNA samples.
For lower throughput projects customers should consider TaqMan
Platform
- MATRIX PlateMatePlus for high throughput liquid handling.
- SNPlex 48-plex SNP genotyping system for simultaneously assay of large numbers of SNPs.
- Applied Biosystems GeneAmp PCR system 9700, and MJ Research PTC-225 tetrad - PCR
- Applied Biosystems 3730XL DNA analyser.
- GeneMapper v4.0 from Applied Biosystems.
Use Geneservice because...
- Quality: Rapid turnaround and accurate results. All data is sent in strictest
confidence to you on CD-ROM, e-mail or web server.
- Pricing: Our high throughput reduces unit labour and capital costs meaning we can
offer exceptional value for money. Furthermore, savings are made by bulk purchase of reagents
and discount deals with suppliers.
- Commitment to improvement: We constantly review and implement new technologies to
reduce costs, shorten timelines and reduce consumables including amounts of your DNA.
Contact
E-mail: Genotyping Team
Tel: +44(0)1223 432608 |
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