Microsatellite Genotyping

Description

Microsatellites are repetitive sequence elements – usually a dinucleotide such as (CA)n. There are tens of thousands of microsatellites in the human genome. Any one microsatellite locus will have a number of different alleles, each with a different number of repeat units.

We have earned a strong reputation in microsatellite typing. Our present operations evolved from the highly regarded ‘HGMP Linkage Hotel’, and we now offer high throughput contract research as well as data analysis.

Application

Microsatellite typing has been used in thousands of linkage analyses of Mendelian traits to detect co-segregation of a distinct phenotype with a particular microsatellite allele in a family. It has also been used to identify genes in complex diseases.

• Geneservice has optimised protocols for using ABI PRISM® Linkage Mapping Sets. These are sets of microsatellite markers distributed at medium density (10 centiMorgan) and high density (5cM) across the human genome.
• Geneservice also designs, validates and applies user-defined microsatellites.

Platforms

• MATRIX Platemate Plus for high throughput liquid handling.
• ABI Prism 3730 and 3100 Genetic Analysers for high throughput PCR product analysis
• Automated data analysis

Why use Geneservice?

• Quality: Backed by nearly ten years experience and with a track record of successes in detecting linkage, our contract research is fast and accurate.
• Pricing: Our high throughputs reduce unit labour and capital costs meaning that we can offer exceptional value for money. Furthermore, savings are made by bulk purchase of reagents.
• Personal Touch: We work with each customer to ensure optimisation of experimental design. All data is sent in strictest confidence to you on CD-ROM (or e-mail if preferred).
• Commitment to improvement: We are continuously introducing new technologies to reduce costs, shorten timelines and reduce consumables including your DNA.

Publications

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