Next Generation Sequencing with Illumina Genome Analyzer and its Applications

Description

As a leading provider of genomic research service in the UK, Geneservice is pleased to offer the Illumina Genome Analyzer technology.

Genome Analyzer Technology

Based on revolutionary Solexa® Sequencing technology, the Illumina Genome Analysis system is a groundbreaking new platform for genetic analysis and functional genomics. It dramatically improves speed and reduces costs. Leveraging proprietary reversible terminators and Clonal Single Molecule Array technology, the Illumina Genome Analysis system has the potential to generate upwards of one to three billion bases of data per run, and in the process transform the way many experiments are devised and carried out.

Applications of Genome Analyzer

1. Genome Sequencing and Resequencing
   • Discover and confirm SNPs
   • Identify chromosomal rearrangements, including Copy Number Variations (CNVs)
   • Map break points
   • Detect rare variants
   
   
2. Digital Gene Expression

   Generates genome-wide expression profiles through sequencing and not hybridization.
   Illumina offers two Digital Gene Expression applications:

   • Tag Profiling
     • Tag Profiling produces high-quality quantitative data empowering whole-genome profiling of any polyadenylated mRNA.
     • Sequence knowledge not required - universal platform to study any transcript
   • Small RNA Discovery and Analysis
     • Discovery and profiling of all forms of small non-coding RNA including miRNA analysis
     • Unbiased discovery platform - no prior sequence or secondary structure information required
   
   
3. ChIP-Seq Application

   ChIP-Seq combines chromatin immunoprecipitation (ChIP) with Solexa Sequencing technology to identify and quantify in vivo protein-DNA interactions on a genome-wide scale. Sequencing ChIP-enriched DNA fragments enables identification of binding sites of DNA-binding proteins to precisely and cost effectively map all binding sites for a protein of interest to any sequenced genome.

4. DNA Methylation

   Discover methylation variable positions across the whole genome by sequencing from:

   • Methylation sensitive restriction digests
   • Bisulfite conversion
   • Anti-methyl C precipitation

Bioinformatics

Our Bioinformatics scientists provide a complete analysis package that can also be customised to your specific research needs.

Contact