To complement Geneservice’s every increasing portfolio of cDNA resources, we are pleased
to announce the distribution of a high quality combinatorial cloning system, StarGate®.
Any gene of interest can be systematically transferred into E. coli, yeast, baculovirus and
mammalian expression systems. To enhance your experiment further, optimal promoters, signal
sequences and tag(s) may also be incorporated into your final expression construct.
Introduction
The StarGate® cloning system uses efficient procedures to generate functional recombinant
proteins or protein complexes which are of key importance in state-of-the-art life sciences.
Many tools including various expression hosts (bacteria, yeast, insect and mammalian cells),
promoters, affinity or fluorescent tags are currently available to express, purify, detect or
immobilize recombinant proteins.
Due to the diverse nature of proteins, however, it is impossible to predict which combination
of these tools will perform best. Therefore StarGate® offers rapid and highly efficient
sub-cloning of any gene – initially cloned into a Donor Vector - to simultaneously fuse it with
many different genetic surroundings via transfer into Acceptor Vectors to generate Destination Vectors.
The latter enable the efficient expression of your protein with various features (e.g. different
tags and different promoters) in different hosts.
Standard StarGate® Procedure
Step 1: Entry reaction
In the first step, a gene of interest (GOI) is equipped at both ends with combinatorial sites (4 bases)
by PCR and is inserted into an Entry Vector by a simple one-tube reaction. The opened Entry Vector
donates the recognition sites and provides an operative linkage with the combinatorial sites for the
highly specific StarGate® gene transfer process (step 2).
Step 2: Transfer reaction
After sequence confirmation, the resulting Donor Vector is the source for the highly parallel sub-cloning of
the GOI by a second simple one tube reaction into a multitude of Acceptor Vectors, each providing a
different genetic surrounding like host specific promoters and different purification tags. The resulting
Destination Vectors are then transformed into the corresponding host cells for further experiments.
Acceptor Vectors are listed in the Acceptor Vector Overview Table.
For StarGate® newcomers! We’ve compiled a 50% discount for the "StarGate® Newcomer" Set which provides
all products required to perform the entire StarGate® protocol. Please remember to choose a number and
type of Acceptor Vectors according to your needs and order them separately.
Geneservice are also offering a cloning service using the StarGate® cloning system. If you would
like further information regarding this or would like to discuss your requirements, please contact
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