bMQ Mouse BAC Library

(Clones and plates)

Please note that the clone(s) being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.

THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.

WHAT TO DO WITH YOUR CLONES WHEN YOU RECEIVE THEM:

Plates contain LB broth with 7.5% glycerol and chloramphenicol. The plates are sent on Dry Ice, and should be stored at -70°C

Individual clones pools have been streaked onto LB agar containing chloramphenicol (20 μg/ml). Please store them at 4°C (not in a freezer). As soon as possible, transfer the culture to LB broth containing chloramphenicol + 8 % glycerol and incubate overnight at 37°C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37°C for subsequent freezing at -70°C.

These clones are for research purposes only.

bMQ library, E.coli host strain

Name: DH10B

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG