pRetroSuperCam vector

Clones are streaked onto LB agar containing ampicillin (50µg/ml) and chloramphenicol (20µg/ml). To use: re-streak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight. Clones can be grown in LB broth + ampicillin & chloramphenicol, and glycerol added after growth to a concentration of 25% for long-term storage at -70°C.