Human shRNAi library
Please note that the clone(s) being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.
THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.
WHAT TO DO WITH YOUR CLONES WHEN YOU RECEIVE THEM:
Plates- contain LB broth with 8% glycerol, ampicillin and chloramphenicol, are sent on Dry Ice. These should be stored at -70°C
Individual clones pools have been streaked onto LB agar containing ampicillin (50µg/ml) and chloramphenicol (20µg/ml).
Please store them at 4°C (not in a freezer). As soon as possible, transfer the culture to LB broth containing ampicillin /chloramphenicol + 8% glycerol
and incubate overnight at 37°C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop).
Incubate overnight at 37°C for subsequent freezing at -70°C.
Background strain: DH5 alpha genotype: fhuA2
∆(argF-lacZ)U169 phoA glnV44 F80 ∆(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
These clones are for research purposes only.
More information:
To view the information of the plasmid vector/sequence and database for this resource please visit: Vector/Sequence
Acknowledgements and Subsequent Publication:
We are grateful to Dr. Roderick Beijersbergen (NKI) for the preparation of this library and Dr. Róisín NicAmhlaoibh (CRUK) for the commercialization.
Please acknowledge the NKI, Cancer Research UK and Geneservice in any resulting publications.
Please also cite the following paper:
Bernards R, Brummelkamp TR, Beijersbergen RL. shRNA libraries and their use in cancer genetics. Nat Methods. 2006 Sep;3(9):701-6. Review.
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