C.elegans RNAi - Library Plates and individual Bacterial
Strains
The bacterial strains in the 384-well library plates have been grown in
LB broth containing 8% glycerol and 25µg/ml carbenicillin. The plates
have been shipped to you on dry ice and should be frozen at -70°C
on receipt.
Individual bacterial strains have been streaked onto LB
agar containing 25µg/ml carbenicillin. The agar slopes should be
stored at 4°C on receipt.
If you encounter problems growing clones, please streak
for single colonies onto LB agar containing 50µg/ml Ampicillin and
15µg/ml Tetracycline.
Please see the accompanying Ahringer Lab protocol for use
of this library.
There is also a "
C.elegans Finder" tool available which allows you to find
the primer sequences for any given GenePair Name in addition to the location
of that bacterial strain in Geneservice Ltd. 384-well plates. The complete C.elegans
RNAi database can be downloaded by right
clicking here.
Every effort has been made to ensure that the plates that
you receive are as close as possible to the published database. However
in some plates, there may be empty wells due to a clone not growing -
please inform us if this happens and we will try to replace this clone
if possible. In the last plate of each set, due to plates having been
condensed from 96 well into 384 well plates- there may be only eg only
1 x 96 well plate resulting in 75% of the plate being empty. If any growth
is observed in a well thatshould be empty, please ignore this, it may
be due to very low level cross contamination.
For the re-arrayed subsets please consult the following datasheets
- Chromatin
- Phosphatase
- Transcription factors
C. Elegans RNAi Bacterial
Strains - an explanation of your Despatch Note
On your Despatch Note a Bacterial Strain is called a "CLONE".
Below is an example of a Despatch Note printout indicating the different
IDs used:
Subsequent publication
Please acknowledge the originator of the library, Dr Julie
Ahringer, and Geneservice Ltd for providing the library in any publication
arising out of using this resource.
Devgen Notice
a)
The RNAi feeding library may be provided to not-for-profit and commercial
entities, but for research purposes only.
For any intended commercial use of the RNAi feeding technology, your attention
is drawn to the following patent applications and patents in the name
of Devgen N.V., Belgium: EP-A-1 093 526, EP-A- 1 197 567, GB 2349885-B,
GB 2370275-A and GB-2362885-B, and further patent applications and patents
that may correspond thereto.
Geneservice does not have the right to grant any license (either explicit or implied)
under any patent applications and/or patents held by Devgen N.V.. Should
you wish to obtain such a license, you are advised to contact Devgen N.V.,
tel. Belgium-9-3 24 24 24; fax Belgium-9-3 24 24 25 or e-mail: info@devgen.com
.
b)
The RNAi feeding library is provided for research purposes only, and
may not be used for any commercial purposes, including but not limited
to: (1) screening by any entity other than a not-for-profit organization
of the RNAi feeding library or any substantial part thereof of on average
more than 30 genes per screening day; and/or (2) offering and/or providing
to third parties, in return for any financial and/or other consideration,
of any services relating to and/or involving the RNAi feeding library.
For any intended commercial use of the RNAi feeding technology, your attention
is drawn to the following patent applications and patents in the name
of Devgen N.V., Belgium: EP-A-1 093 526, EP-A- 1 197 567, GB 2349885-B,
GB 2370275-A and GB-2362885-B, and further patent applications and patents
that may correspond thereto.
Geneservice does not have the right to grant any license (either explicit or implied)
under any patent applications and/or patents held by Devgen N.V.. Should
you wish to obtain such a license, you are advised to contact Devgen N.V.,
tel. Belgium-9-3 24 24 24; fax Belgium-9-3 24 24 25 or e-mail: info@devgen.com
.
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