Sequence Verified C. elegans RNAi Clones
Geneservice will end sequence your clones before they are sent to
you - you only pay for the clones that BLAST match with the
id you requested. This risk free option saves you time when you get your
clone and removes the chance of receiving one of the wrong clones that
are inherent in all copies of large libraries.
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- Place your order conventionally but follow the Sequence Verified clones link
- Geneservice will extract DNA, end sequence your requested clone(s) and BLAST
the output
- Successfully verified clones (>95% identity after quality clipping) will be shipped to you and we will include a BLAST report in the packet
- Customers will be notified in their delivery about the clones that
fail verification, no charge will be made for failed clones. In the unlikely
event that all clones in the order fail verification the order will be
completely cancelled at no charge and customers will be contacted by email.
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C. elegans RNAi library
This Caenorhabditis elegans RNAi feeding library provided
by Geneservice was constructed by Julie Ahringer's group
at the The Wellcome CRC Institute, University of Cambridge,
Cambridge, England. .
C. elegans genomic fragments were PCR
amplified using Research
Genetics GenePairs, cloned into the EcoRV site of vector
L4440 from Timmons and Fire (Nature,
395, 854) and transformed into bacterial strain HT115
(Gene,
263, 103-112) as described (Nature
408, 325-330). The whole genome library consists of 16,757
bacterial strains, which cover 87% of C. elegans genes.
Bacterial strains carry the GenePairs name, which usually,
but does not always correspond to a predicted C. elegans
gene name. A current mapping of GenePair to gene can be found
in WormBase (http://www.wormbase.org).
GenePairs primer sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.
There is also a "C.elegans
Finder" tool available which allows you to find the primer sequences
for any given GenePair Name in addition to the location of that bacterial
strain in Geneservice 384-well plates. The complete C.elegans RNAi
database can be downloaded by
right clicking here . Note that the mapping of some GenePairs has changed since WS56 when this database was built. A correlation table between
WS56 and WS152 can be accessed here.
The libraries is available by individual chromosome sets (I, II, III,
IV, V and X) from Geneservice as frozen glycerol stocks of bacterial
strains arrayed in 384 well plates or as individual bacterial strains
(clones).
We are also supplying re-arrayed sub-sets of the libraries in the following areas:
- Chromatin (257 clones)
- Phosphatase (166 clones)
- Transcription factors (387 clones).
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