C. elegans ORF-RNAi Library v1.1
This ORF-based RNAi library is ideal for either high-throughput screening or individual knockdown experiments.
The ORFeome-RNAi v1.1 library contains 11,511 RNAi clones, which targets 10,953; 485 of these genes are targeted by 2 or more constructs. The library therefore covers the potential knockdown of 55% of the 19,920 unique protein-coding genes predicted in "worm sequence" 112.
Interestingly, there are ~1,700 unique genes not previously targeted by existing RNAi feeding libraries (an additional ~9% of the worm genome). Therefore, in conjunction with our exiting RNAi feeding library (http://www.geneservice.co.uk/products/rnai/Celegans.jsp) the overall predicted coverage of worm genes is ~86%.
Format:
Geneservice offers individual clone(s) for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact biohelp to discuss your requirements.
Reference:
Rual JF, Ceron J, Koreth J, Hao T, Nicot AS, Hirozane-Kishikawa T, Vandenhaute
J, Orkin SH, Hill DE, van den Heuvel S, Vidal M. "Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library". Genome Res. 2004 Oct;14(10B):2162-8.
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber
Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
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