C. elegans RNAi library
This Caenorhabditis elegans RNAi feeding library provided
by Geneservice Ltd was constructed by Julie Ahringer's group
at the The Wellcome CRC Institute, University of Cambridge,
Cambridge, England. .
C. elegans genomic fragments were PCR
amplified using Research
Genetics GenePairs, cloned into the EcoRV site of vector
L4440 from Timmons and Fire (Nature,
395, 854) and transformed into bacterial strain HT115
(Gene,
263, 103-112) as described (Nature
408, 325-330). The whole genome library consists of 16,757
bacterial strains, which cover 87% of C. elegans genes.
Bacterial strains carry the GenePairs name, which usually,
but does not always correspond to a predicted C. elegans
gene name. A current mapping of GenePair to gene can be found
in WormBase (http://www.wormbase.org).
GenePairs primer sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.
There is also a "C.elegans
Finder" tool available which allows you to find the primer sequences
for any given GenePair Name in addition to the location of that bacterial
strain in Geneservice Ltd. 384-well plates. The complete C.elegans RNAi
database can be downloaded by
right clicking here . Note that the mapping of some GenePairs has changed since WS56 when this database was built. A correlation table between
WS56 and WS152 can be accessed here.
The libraries is available by individual chromosome sets (I, II, III,
IV, V and X) from Geneservice Ltd as frozen glycerol stocks of bacterial
strains arrayed in 384 well plates or as individual bacterial strains
(clones).
We are also supplying re-arrayed sub-sets of the libraries in the following areas:
- Chromatin (257 clones)
- Phosphatase (166 clones)
- Transcription factors (387 clones).
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