Human Domain Antibody Library (DAb library)

Geneservice™ is pleased to announce the availability of the Domain Antibody Phagemid library constructed by Dr Daniel Christ (formally of the MRC Laboratory of Molecular Biology, Cambridge UK and currently based at the Garvan Institute, Sydney, Australia).

This artificial library of antibodies can be used to derive binders to almost any target molecule using phage display and selection. These binders can be used for the same applications as conventional monoclonal antibodies (ELISA, Western blotting, FACS, immunohistochemistry), but can be isolated in a fraction of the time and without the need for animal immunisation.

Phage-antibody libraries have been used successfully in hundreds of molecular biology labs worldwide to derive highly specific antibody reagents to a wide range of different proteins, peptides or small molecule compounds

This library is based on a VH framework (V3-23/D47). Diversity was introduced into the antigen binding domains by PCR mutagenesis into CDR1, CDR2 and CDR3. There are many benefits to this library.

• Produced binders to the model antigens refold without major loss of antigen-binding activity upon cooling.
• Antibodies selected maintain antigen-binding activity after up to 25 cycles of heat denaturation, rivalling the heat resistance of thermophilic protein such as Taq polymerase.
• Repertoire withstands heat-induced aggregation on phage.

These benefits offer a wide range of diagnostic and therapeutic research applications and can further maximise the number of binders to your antigen of interest.

• Phagemid antibody library (~3x10 9 )
• Helper phage KM13
• Host strain TG1Tr
• Optimised antibody expression strain HB2151
• Controls: anti-beta-galactosidase & anti-bovine ubiquitin antibodies
• Detailed protocol

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