ATLAS scFV Constructs

To enhance our proteomic resources Geneservice are now distributing the ATLAS clones constructed and annotated at the Welcome Trust Sanger Institute by team 86.

Antibodies were selected against 404 antigen targets which represent 280 mouse genes. The antigens were primarily produced in E.coli or within mammalian expression system; the majority of these genes are cell surface receptors.

The scFv constructs are fused to a tri-FLAG tag sequence and the dimeric bacterial enzyme, alkaline phosphatase. This has a dual rationale of driving dimerisation which increases avidity of binding and also acts as a useful enzymatic tag for detection purposes. 22 unique clones were selected for each antigen. Antibodies were characterised by specificity testing and detailed sequence analysis. Furthermore, many of scFvs have been used in immunohistochemistry analysis on tissue microarrays.

Geneservice Ltd offers the complete clone set of 84 96-well microtitre plates or individual clones, for research purposes only. For each antigen, 22 unique scFv constructs are available.

We can also make subsets of your chosen clones from the collection. Please contact biohelp to discuss your requirements.

Please utilise the AtlasDB antibody database to search for your desired scFv constructs.