Subsets of the I.M.A.G.E. Consortium resources

The I.M.A.G.E. resources are comprised of a large number of arrays of cDNA libraries from RNA made on different species and tissues. Six subsets of clones from these arrays have been picked and are available.

The Mammalian Gene Collections (two human, two mouse and two rat).
This is described here.

The Xenopus Gene Collections (two X. laevis, and one X. tropicalis).
This is decribed here.

The Zebrafish Gene Collection.
This is decribed here.

The Mouse Lymphochip subset
I.M.A.G.E. Consortium calls this subset IRAP. The clones were selected on the basis of an association with lymphoid tissue. The subset contains 12,480 different clones representing 12,480 genes. The clones have not been fully sequenced.

The inserts are harboured in ampicillin-resistant vectors. There are 130 (96-well) plates. On any one plate, just one vector has been employed. This resource is distributed as a single set.
The identity of the clones is given here.

The Human Lymphochip subset
The I.M.A.G.E. Consortium calls this subset IRAY, IRBA, IRAZ and IRBB. The clones were selected on the basis of an association with lymphoid tissue. The subset contains 2,517 different clones. The clones have not been fully sequenced.
The subset contains one representative of 313 genes. The subset contains two representative clones for 1,102 genes.

The inserts are harboured in ampicillin-resistant vectors for the plates in the IRAY and IRBA sets. The inserts are harboured in chloramphenicol-resistant vectors for the plates in the IRAZ and IRBB sets. There are 35 (96 well) plates. On any one plate, just one vector has been employed. This resource is distributed as a single set.
IRAY - 96-well plates numbered 1 - 17 (1280 clones)
IRBA - 96-well plates numbered 1 - 12 (967 clones)
IRAZ - 96-well plates numbered 1 - 3 (135 clones)
IRBB - 96-well plates numbered 1 - 3 (135 clones)
The identity of the clones is given here.

The Human glioma-related subset
The I.M.A.G.E. Consortium calls this subset IRAX. The clones were selected on the basis of an association with gliomas. These are a diverse group of brain tumors that arise from the normal glial cells of the brain. The subset contains 5,664 clones usually representing different genes. The clones have not been fully sequenced.

The inserts are harboured in ampicillin-resistant vectors. There are 59 (96 well) plates. On any one plate (except plate 1), just one vector has been employed. Plate 1 contains three different vectors. This resource is distributed as a single set.
The identity of the clones is given here.

Mouse pancreas genes:
The I.M.A.G.E. Consortium calls this subset IRBD and IRBE. This set of 7000 mouse genes (IRBD (amp-resistant) and IRBE (kanamycin-resistant) has been rearrayed by the K. Kaestner laboratory (University of Pennsylvania) in 96-well format. Each plate contains clones from one vector only. There are 4 partially-full plates.A flatfile containing all the data (IRBD_IRBE.preSV.data) can be found on the anonymous ftp site at ftp://image.llnl.gov/image/rearrayed_plates/IRBD_IRBE.preSV.data.This resource is available as a single set. Individual clones can be ordered on our standard IMAGE order form.