Sequence Verified I.M.A.G.E. Consortium cDNA Clones
Geneservice will end sequence your clones before they are sent to
you - you only pay for the clones that BLAST match with the I.M.A.G.E.
id you requested. This risk free option saves you time when you get your
clone and removes the chance of receiving one of the wrong clones that
are inherent in all copies of the I.M.A.G.E library.
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- Place your order conventionally but follow the Sequence Verified I.M.A.G.E.
clones link
- Geneservice will extract DNA, end sequence your requested clone(s) and BLAST
the output
- Successfully verified clones (>95% identity after quality clipping) will be shipped to you and we will include a BLAST report in the packet
- Customers will be notified in their delivery about the clones that
fail verification, no charge will be made for failed clones. In the unlikely
event that all clones in the order fail verification the order will be
completely cancelled at no charge and customers will be contacted by email.
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Image Consortium
The I.M.A.G.E. consortium
make high quality, arrayed cDNA libraries available to the
community. Each clone has been given a unique I.M.A.G.E. ID
to facilitate reference. The sequence, mapping and expression
data has been placed in the public domain. These clones are
available free of any royalties and are available to anyone
agreeing with their guidelines. Lennon et al. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=96224170&dopt=Citation
Mammalian Gene Collection
The Mammalian Gene Collection
(MGC) is an NIH initiative to generate full-length cDNA resources.
Geneservice is now able to supply these full-length cDNA
clones. Click here for more information.
- Fully-sequenced clones have been sequenced throughout the coding region of the gene, by the IMAGE consortium.
- Partially sequenced clones have gone through a single round of sequencing either the 3’ or 5’ coding region of the gene, by the IMAGE consortium.
Image Consortium 8-digit clones
Due to the high levels of redundancy in the human and mouse
MGC collections, clones from those species arrayed for 5'
sequencing since October 2002 (production plates LLAM, LLCM,
and LLKM) will no longer be routinely available. Please note
the following changes, effective October 2002:
- All human and mouse MGC clones have an 8-digit IMAGE identifier
to distinguish them from clones arrayed prior to this date,
which have 5, 6, or 7 digit identifiers.
- Only human and mouse clones sent out for full-insert sequencing
are available from all IMAGE distributors on a routine basis
(as before). To see if a particular clone is included in
the completely sequenced set, please check ftp://image.hudsonalpha.org/image/outgoing/ND_available_clones.txt.
- Human and mouse MGC clones not sent out for full-insert
sequencing and which are non-redundant (one whose sequence
is unique, i.e. no other clone from the same species contains
an identical sequence) can still be ordered directly from
the IMAGE distributors, but will require additional processing
time.
Geneservice will check if these can be obtained and if
so, we will order them for you for our normal clone prices
plus an Admin charge of GBP 25 per clones. Please be aware
that due to the increased complexity in checking and eventually
getting these clones, it will take a minimum of 3 weeks for
an order to be processed.
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