I.M.A.G.E. Clone Availability

Geneservice Ltd has most clones available from the following range of I.M.A.G.E. ID's:

This table was last updated: 17th October 2007

If you use our on-line WWW request form to order an I.M.A.G.E. clone from within this available range and find that your request is not accepted it means that the clone is NOT available from Geneservice Ltd.

There are three main reasons for clones not being available:

Phage Contamination

There are now only a few clones that are unavailable due to phage contamination, as we have now received replacement, phage-tested plates from the I.M.A.G.E. Consortium for nearly all the contaminated original plates.

Plates Not Yet Received

We have not yet received all the plates for the current range of I.M.A.G.E. IDs. In the past we have provided a list of the plates not received and their associated I.M.A.G.E. IDs. Recently the I.M.A.G.E. Consortium have changed the way they assign IDs to clones, and a single plate does not now necessarily have 384 contiguous IDs assigned to it. This resulted in our list becoming too long to publish, but the order request tool searches this list for you. It also means that we may not have in stock all clones with IDs lower than the ID shown in the table above.

8 Digit Numbers

Due to the high levels of redundancy in the human and mouse MGC collections, clones from those species arrayed for 5' sequencing since October 2002 (production plates LLAM, LLCM, and LLKM) will no longer be routinely available from the IMAGE Consortium Distributors. Please note the following changes, effective October 2002: - All human and mouse MGC clones have an 8-digit IMAGE identifier to distinguish them from clones arrayed prior to this date, which have 5, 6, or 7 digit identifiers. - Only human and mouse clones sent out for full-insert sequencing are available from all IMAGE distributors on a routine basis (as before). To see if a particular clone is included in the completely sequenced set, please check ftp://image.llnl.gov/image/outgoing/ND_available_clones.txt. - Human and mouse MGC clones not sent out for full-insert sequencing and which are non-redundant (one whose sequence is unique, i.e. no other clone from the same species contains an identical sequence) can still be ordered directly from the IMAGE distributors, but will require additional processing time. Please send requests to our Enquries helpdesk, we will then see if these can be obtained and if so, we will order them for you for our normal clone prices plus an Admin charge of 25 pounds per clone.

Please be assured that we receive regular consignments of I.M.A.G.E. plates and endeavour to replicate them and make the clones available as promptly as possible. Whenever new clones are made available our database is adjusted to allow these clones to be ordered from us. We do not however know in advance which clones we will receive and are unable to predict a delivery date for any particular clone. Our advice is that if you try and request a clone and it is not available then try repeating your request at a later date.

^* Antibiotic:
Most I.M.A.G.E. clones are grown in ampicillin (AM) - we do not pre-fix these clones on our despatch note. Some of the I.M.A.G.E. clones from the Mammalian Gene Collection are grown in chloramphenicol (CM) and the plate numbers start from 1 - we pre-fix these clones with CM on our despatch note. In addition there are some plates grown in kanamycin (KM) with plate numbers again starting at 1 - we pre-fix these clones with KM on our despatch note.

Please note that if the I.M.A.G.E. CloneID is not apparent and if there is no mention of the I.M.A.G.E. Consortium in the database entry then it is NOT an I.M.A.G.E. clone and Geneservice Ltd does NOT have the clone.
How to identify the I.M.A.G.E. ID

WARNING: Life Tech. Adult brain (mouse) library

This library, which is part of the I.M.A.G.E. Consortium library has been found to be problematic. It is probably of rat origin.
More information can be obtained on:

http://www.ncbi.nlm.nih.gov/dbEST/warning.html

All dbEST sequence entries from this library have been withdrawn.