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WHAT TO DO WITH YOUR PLATES WHEN YOU RECEIVE THEM
IRAY and IRBA
Plates have been replicated in LB broth containing 8% glycerol and 50µg/ml
ampicillin.
17 IRAY plates and 12 IRBA plates (96-well format) have been shipped frozen
in dry ice and should be placed in a -70°CC freezer on arrival.
To make further copies replicate in the above media and incubate overnight
at 37°C.
IRAZ and IRBB
Plates have been replicated in LB broth containing 8% glycerol and 27µg/ml
chloramphenicol.
3 IRAZ plates and 3 IRBB plates (96-well format) have been shipped frozen
in dry ice and should be placed in a -70°C freezer on arrival.
To make further copies replicate in the above media and incubate overnight
at 30°C.
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These frozen stocks can be re-grown for plasmid isolation and purification
or PCR amplification.
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