The Zebrafish Gene Collection
The Zebrafish Gene Collection
(ZGC) project is an NIH initiative which was launched in April 2002,
as a subproject of the existing Mammalian Gene Collection.
The general approach is to acquire or construct high-quality cDNA libraries.
The libraries are screened by sequencing the 5' end of the clones.
Putative full open reading frames are identified, and finally, selected clones
are full-length sequence verified. The goal of the project is to make
available a complete set of these full-length cDNA clones in Zebrafish.
The ZGC plan of work is a phased project:
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Initially, the ZGC will collect 5' sequences from clones in various libraries over a 3-month period.
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At the end of that time, an external panel of scientists will review the list of all the putative full-length clones eligible for full-length sequencing and prioritize those for sequencing.
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This process will then be repeated quarterly.
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In addition, the ZGC will collaborate with other researchers who have significant existing collections of putative full-length clones to sequence those clones.
In summary a Zebrafish Gene Collection (ZGC) clone is identified by:
Description of rearrayed plates containing sequenced full-length
MGC clones:
| IRBO | Zebrafish | Vectors
confer ampicillin resistance |
| IRBV | Zebrafish | Vectors
confer chloramphenicol resistance |
NB: In each of these subsets all clones on one plate share the same vector
(IRBO, IRBV).
Details of the vectors used can be found here
I.M.A.G.E. has a clone and plate query tool
which allows you to obtain all the current information for any ZGC plate -
IMAGE ID, ZGC_ID, species, vector, rearray clone location, Genbank Accession number, Unigene Cluster Number and
GenBank definition line and gene symbol.
Also available from Geneservice Ltd, CloneHunter™ can help you to quickly find the clones you need to advance your research via BLAST matches or by the means of various genes identifiers.
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During a recent sequencing effort, the IMAGE consortium has produced QC statistics for some of the rearrayed libraries (click here)
Clones from this subset can be ordered as individual clones (streaks on agar) and 96-well plates (frozen bacterial
stocks in glycerol).
Clones are ordered using the IMAGE ID on the standard I.M.A.G.E. order
form (you cannot use the ZGC_ID). If the clone ordered forms part of a ZGC
subset in the Geneservice Ltd collection, you will receive the clone streaked out
from IRBO and IRBV plates.
Such clones will also have an ZGC_ID which will be given on the email
confirmation of your order. If a clone has an ZGC_ID but is not yet part of
the subset plates received by Geneservice Ltd the clone will be streaked out from the
main I.M.A.G.E. collection.
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