The Xenopus Gene Collection
The Xenopus Gene Collection (XGC) project is trans-NIH initiative,
which was launched, as a subproject of the existing Mammalian Gene Collection.
The general approach is to acquire or construct high-quality cDNA libraries.
The libraries are screened by sequencing the 5' end of the clones.
Putative full open reading frames clones are identified, and finally,
selected clones are full-length sequence verified. The goal of the project is to make
available a complete set of these full-length cDNA clones in Xenopus laevis and Xenopus tropicalis.
The XGC plan of work is:
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The generation of libraries from different stages of development and adult tissues.
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Sequencing of ~3,000 to 10,000 5' and 3' ESTs from each library.
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All sequences are deposited into GenBank. The corresponding clones are available through the IMAGE clone distribution network and are fully accessible to the community.
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Analysis of all available sequences into UniGene clusters.
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Selection of candidate complete open reading frame (ORF) clones and their sequencing.
In summary a Xenopus Gene Collection (XGC) clone is identified by:
Description of rearrayed plates containing sequenced full-length
MGC clones:
| IRBH | X. laevis | Vectors
confer ampicillin resistance |
| IRBG | X. laevis | Vectors
confer chloramphenicol resistance |
| IRBN | X. tropicalis | Vectors
confer ampicillin resistance |
NB: In each of these subsets all clones on one plate share
the same vector (IRBH,
IRBG and IRBN).
Details of the vectors used can be found
here
I.M.A.G.E. has a clone and plate query tool which allows you to obtain all the current information for any XGC plate - IMAGE ID, XGC_ID, species, vector, rearray clone location, Genbank Accession number, Unigene Cluster Number and GenBank definition line and gene symbol.
Also available from Geneservice Ltd, CloneHunter™ can help you to quickly find the clones you need to advance your research via BLAST matches or by the means of various genes identifiers.
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During a recent sequencing effort, the IMAGE consortium has produced QC statistics for some of the rearrayed libraries (click here)
Clones from the three subsets can be ordered as individual clones (streaks on agar) and 96-well plates (frozen bacterial
stocks in glycerol).
Clones are ordered using the IMAGE ID on the standard I.M.A.G.E. order
form (you cannot use the XGC_ID). If the clone ordered forms part of a XGC
subset in the Geneservice Ltd collection, you will receive the clone streaked out
from IRBH, IRBG, or IRBN plates.
Such clones will also have an XGC_ID which will be given on the email
confirmation of your order. If a clone has an XGC_ID but is not yet part of
the subset plates received by Geneservice Ltd the clone will be streaked out from the
main I.M.A.G.E. collection.
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