The Mammalian Gene Collection
The Mammalian Gene Collection
(MGC) project is an NIH initiative. The goal of the project is to make available
a complete set of these full-length cDNA clones in human, rat,
mouse and cow.
The MGC plan of work is:
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To produce libraries enriched for full-length cDNA;
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To sequence the 5' and 3' ends of 768 clones in each
library for library validation;
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To array and sequence from each validated library the
5' ends of 10,000 to 30,000 clones. Each of these clones
is given an I.M.A.G.E. ID and becomes part of the main
I.M.A.G.E. resource (LLAM and LLCM clones);
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To apply several algorithms to analyze the 5' sequences
to identify clones containing putative N-terminal coding
regions. The clones identified are rearrayed into 384-well
plates by the I.M.A.G.E.
Consortium at LLNL. These plates are labelled with
the prefix IRAK, IRAL or IRAM on the I.M.A.G.E.
web site and each clone retains its unique IMAGE ID;
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Only those clones confirmed by sequencing
to harbour full-length ORFs are assigned a unique MGC
identifier (e.g. MGC_15287 or its synonym MGC:15287) in
addition to their original IMAGE ID. These sequences are
deposited in GenBank.
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To rearray clones with a unique MGC_ID into 96-well plates.
In summary a Mammalian Gene Collection (MGC) clone is identified
by:
Description of rearrayed plates containing sequenced full-length
MGC clones:
| IRAT | Human | Vectors
confer ampicillin resistance |
| IRAU | Human | Vectors
confer chloramphenicol resistance |
| IRCM | Human | Vectors
confer Kanamycin resistance |
| IRAV | Mouse | Vectors
confer ampicillin resistance |
| IRAW | Mouse | Vectors
confer chloramphenicol resistance |
| IRCL | Mouse | Vectors
confer Kananmycin resistance |
| IRBP | Rat | Vectors
confer ampicillin resistance |
| IRBQ | Rat | Vectors
confer chloramphenicol resistance |
| IRCJ | Cow | Vectors
confer amplicillin resistance |
NB: In each of these subsets all clones on one plate share
the same vector (IRAT,
IRAU, IRCM, IRAV, IRAW, IRCL, IRBP, IRBQ and IRCJ). Details of the vectors used can be found
here
Only available on the Geneservice website, the
MGC
Finder is provided to enable you to obtain all
the current information on an MGC clone - IMAGE ID,
MGC_ID, species, vector, rearray clone location, Genbank
Accession number, Unigene Cluster Number, GenBank
definition line and gene symbol.
In addition, MGC Finder now has a plate query tool
which allows you to obtain all the above information
for any MGC plate.
Also available from Geneservice, CloneHunter™ can help
you to quickly find the clones you need to advance your research.
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During a recent sequencing effort, the IMAGE consortium has produced QC statistics for some of the rearrayed libraries (click here)
Clones from the six subsets can be ordered as individual clones (streaks on agar) and 96-well plates (frozen bacterial
stocks in glycerol).
Clones are ordered using the IMAGE ID on the standard I.M.A.G.E. order
form (you cannot use the MGC_ID). If the clone ordered forms part of a MGC
subset in the Geneservice collection, you will receive the clone streaked out
from IRAT, IRAU, IRCM, IRAV, IRAW, IRCL, IRBP, IRBQ and IRCJ plates.
Such clones will also have an MGC_ID which will be given on the email
confirmation of your order. If a clone has an MGC_ID but is not yet part of
the subset plates received by Geneservice the clone will be streaked out from the
main I.M.A.G.E. collection.
For a list of the current plates available in each MGC subset please click
here
Publications
Strausberg RL, Feingold EA, Klausner RD & Collins FS(1999). The Mammalian Gene Collection. Science 286: 455-457
MGC (Mammalian Gene Collection) Program Team (2002). Generation and Initial Analysis of more than 15000 Full-Length Human and Mouse cDNA
sequences. PNAS 99(26): 16899-16903
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