Human Cystic Fibrosis Clone Collection
CF is a disorder of the cells that line the lungs, small intestines,
sweat glands and pancreas. Sticky, thick mucus contributes to
the destruction of lung tissue and impedes gas exchange in the
lungs. It also prevents nutrient absorption in the small intestines,
and blocks pancreatic ducts from releasing digestive enzymes.
Approximately 85% of all people with CF cannot properly digest
their food without supplemental enzymes.
A collection of ~52,000 human CF clones, produced by Bento Soares,
is now available from Geneservice Ltd providing a valuable resource
for Cystic Fibrosis research. The clones have been constructed
using a variety of tissues, viz., primary lung epithelial cells,
normal lung tissue, primary lung cystic fibrosis epithelial
cells and lung epithelial cells.
All clones have been cloned directionally into pT7T3-Pac
vector and propagated in E. coli DH10B cells (T1 phage resistant).
This resource is available as bacterial glycerol stocks and
can be ordered as individual clones, individual 384-well plates
or complete set of 163 384-well plates.
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