LLNL HUMAN SINGLE CHROMOSOME LIBRARIES

In many cases, the individual chromosome was flow-sorted from a rodent/hybrid cell line containing the single human chromosome of interest (Trask et al 1991 Som, Cell Mol. Gen. 17 117-136). The flow sorting was done using a high-speed modular flow sorter designed and built by LLNL engineers. Details on the methods used to produce these libraries have been published: (Gingrich et al 1996 Construction and Characterization of Human Chromosome-2-Specific Cosmid, Fosmid and PAC Clone Libraries. Genomics 32 65-74).

Further Information can be obtained from LLNL

Phage Contamination - Please Read

The LLNL libraries exist both in a 96-well plate and a 384-well plate format, and it is essential that there is no confusion in the databases (e.g. GDB) between clones identified from these different formats.

The LLNL libraries now at Geneservice were obtained in a 384-well format in June 1996. Prior to that time they only existed in the 96-well format. Consequently all database entries and publications are in the 96-well format.

It has been decided that this will be continued so that there is no ambiguity in the identity of LLNL clones.

Therefore please regard the coordinates that you give to your clones when requested as a result of screening filters provided by Geneservice as CODE NAMES ONLY.

When we receive your request we will translate your code names into the correct CLONE NAMES (i.e. the 96 well coordinates). It is these CLONE NAMES that should be used in all publications and database entries.

We can only be sure that we are providing you with the correct clone if we know how you identified the clone ID. For this reason, it is essential that you complete the section on our request forms asking you for the source of your clone ID.

If users wish to convert from 96 to 384 well coordinates (and vice versa) they can use this utility.

Clones from the three Chromosome 19 libraries, F, R and W, should continue to be referred to in publications or database entries by either the LLNL internal ID or the GenBank Clone Name. For a full description of the nomenclature see click here.

Please regard the coordinates that you give to your clones when requested as a result of screening filters provided by Geneservice as CODE NAMES ONLY.

When we receive your request we will translate your code names into the correct CLONE NAMES (i.e. the 96 well coordinates). It is these CLONE NAMES that should be used in all publications and database entries. On your despatch note the CLONE NAME we will use will be in the GenBank format (e.g. LLNLR-310G1) but when submitting data it would be helpful to include the LLNL internal ID (e.g. R34737) in addition.

We provide a tool for correlating all the IDs for a given Chromosome 19 clone at Chromosome19_Finder