LANL HUMAN SINGLE CHROMOSOME LIBRARIES
| Human Chromosome |
Full Library Name |
Library Short Name |
Vector |
Bacterial Host |
Percentage human positive clones |
Percentage non-recombinant clones |
Insert Size |
Number of Clones |
Aprox. Coverage |
| 4 |
LA04NC01 |
LA04 |
sCOS1 |
HB101 |
84$ |
1 |
29kb$ |
22,700 |
3x |
| 5 |
LA05NC01 |
LA05 |
sCOS1 |
DH5alphamcr |
81 |
3 |
35 - 40kb |
22,000 |
4x |
| 6 |
LA06NC01 |
LA06 |
sCOS1 |
HB101 |
n/a |
7 |
35 - 40kb |
26,800 |
4x |
| 8 |
LA08NC01 |
LA08 |
sCOS1 |
DH5alphamcr |
86 |
3 |
35 - 40kb |
20,000 |
4x |
| 10 |
LA10NC01 |
LA10 |
sCOS1 |
DH5alphamcr |
81 |
<1 |
35 - 40kb$ |
22,600 |
5x£ |
| 11 |
LA11NC01 |
LA11 |
sCOS1 |
DH5alphamcr |
83 |
4 |
33kb& |
23,500 |
3.5x& |
| 13 |
LA13NC01 |
LA13 |
sCOS1 |
n/a |
93 |
1 |
35 - 40kb |
23,000 |
7x |
| 14 |
LA14NC01 |
LA14 |
sCOS1 |
DH5alphamcr |
88 |
0.1 |
35 - 40kb$ |
16,800 |
5x |
| 15 |
LA15NC01 |
LA15 |
sCOS1 |
DH5alphamcr |
86 |
<1 |
35 - 40kb |
17,100 |
6x |
| 17 |
LA17NC01 |
LA17 |
sCOS1 |
HB101 |
92 |
6 |
35 - 40kb |
15,300 |
5x |
| 20 |
LA20NC01 |
LA20 |
sCOS1 |
DH5alphamcr |
81* |
<1* |
35 - 40kb |
10,200 |
3x |
$ Data from Riess et al Cytogenet. Cell Genet.
1994 65 : 238-242
£ Data from Ma et al Cytogenet. Cell Genet.
1996 74 : 266-271
& Data from Weber et al Chromosome Research
1994 2 : 201-207
* Data from Stokkae et al Genomics 1995 26
: 134-137
Important information on requesting clones from LANL cosmid
libraries:
The LANL libraries exist both in a 96-well plate and a 384-well
plate format, and it is essential that there is no confusion
in the databases (e.g. GDB) between clones identified from
these different formats. It has been decided that all databases
will continue to be in the 96-well format, so that there is
no ambiguity in the identity of LANL clones.
The LANL libraries now at Geneservice were obtained in
a 384-well format in June 1996. Prior to that time they only
existed in the 96-well format. Consequently all database entries
and publications are in the 96-well format.
Therefore, when requesting clones identified as a result
of screening our filters, please regard the clone co-ordinates
as code names only. When we receive your request we will translate
your code names into the correct clone names (i.e. the 96
well coordinates) which should be used in all publications
and database entries.
For the same reason, it is essential that you always tell
us how the clone you requested was identified, so that there
can be no ambiguity about the clone ID. Please make sure that
you always complete the section on our request form asking
for this information.
If users wish to convert from 96 to 384 well coordinates
(and vice versa), they can use this utility.
Product Data Sheets
(Technical information as supplied with order)
|
Filter Information
|
4
|
5
|
6
|
8
|
10
|
11
|
13
|
14
|
15
|
17
|
20
|
|
Interpretation of Hybridisation Results
|
4
|
5
|
6
|
8
|
10
|
11
|
13
|
14
|
15
|
17
|
20
|
|
Interpretation Sheets
|
4
|
5
|
6
|
8
|
10
|
11
|
13
|
14
|
15
|
17
|
20
|
|
Clones
|
All
LANL Libraries (4, 5, 6, 8, 10, 11, 13, 14, 15, 17, 20) |
|
Explanation of Despatch Note
|
All
LANL Clones |
|