Drosophila Cosmid Library

This Drosophila melanogaster cosmid library was constructed by Inga Siden-Kiamos at the Institute of Molecular Biology and Biotechnology, Heraklion, Greece and supplied by David Glover from the University of Dundee. It has an estimated average insert size of 35kb and contains approximately 18,000 clones providing a 4-fold coverage of the genome. The vector used is Lorist 6 (Gibson. TJ. et al [1987] Gene 53: 283-286). The library consists of 48 plates labelled 1- 50 (plates 45 and 46 are missing).

The library is available from the Resource Centre as one high density gridded double spotted filter (22.2 x 22.2 cm).

Siden-Kiamos. et al [1990]. "Towards a physical map of the Drosophila melanogaster genome: mapping of cosmid clones within defined genomic divisions", Nucl. Acids Res. 18, 6261-6270.

Important information on requesting clones from the Drosophila cosmid library:

This library exists both in a 96-well plate and a 384-well plate format, and it is essential that there is no confusion in the databases (e.g.flybase ) between the different formats. It has been decided that all databases will continue to be in the 96-well format, so that there is no ambiguity in the identity of the Drosophila cosmid clones.

The library held by Geneservice is in the 384 well format. Therefore, when requesting clones identified as a result of screening our filters, please supply the clone coordinates only. When we receive your request we will translate your code names into the correct clone names (i.e. the 96 well coordinates) which should be used in all publications and database entries.

For the same reason, it is essential that you always tell us how the clone you request was identified, so that there can be no ambiguity about the clone ID. Please make sure that you always complete the section on our request form asking you for this information.

If requesting clones from a database entry you can use this utility, to convert from 96 to 384 well coordinates (and vice versa),.