LANL HUMAN SINGLE CHROMOSOME LIBRARIES

Clones

Please note that the clone(s) being sent have tested negative in our phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate

Clones from all the cosmid libraries are supplied streaked onto LB agar + kanamycin (30µg/ml).

Clones on agar should be stored at 4°C on receipt.

As with all clones it is recommended that you re-streak to obtain several single colonies to test. Clones that test positive can be stored long-term as glycerol stocks at -70°C. After growth in LB broth + appropriate antibiotic, glycerol is added to a final concentration of 25%.

sCos-1 Vector (also called SuperCos 1)

Further information on this vector can be obtained from:
http://www.stratagene.com/vectors/selection/cosmid.htm

Subsequent Publication:

Please acknowledge both the originator of the library, LANL as described in the Materials Transfer Agreement that you have signed, and Geneservice for providing the clone, in any publication arising out of using this resource. An example of how a clone (E.g. CLONE NAME = 32-B9 from the Chr. 11 library) should be described in a publication is: LA1132b9 from the LA11NC01 library.

Version 1.2 16th February 2001