Fugu rubripes BAC clones (in E.coli strain DH10B)

Clones are streaked onto LB agar containing chloramphenicol (20 µg/ml).

To use: re-streak the bacteria for single colonies on the same agar type, incubating overnight at 37°C.

Single colonies are then ready for DNA isolation, if required.

Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long term storage at -70°C

pBeloBAC11 Vector

For further information see: http://informa.bio.caltech.edu/protocols/BAC_lib_construction.html

DNA isolation

Included is a protocol which although described for PAC clones also works for BAC clones (with the modification of changing kanamycin for chloramphenicol for the clone growth).

In addition another protocol is supplied. The ProCipitate method is used in house at the Resource Centre with mouse BAC clones and produces DNA which is suitable both for direct sequencing and for PCR assays.

Subsequent publication

Please acknowledge the originator of the library, Dr. Greg Elgar at the UK HGMP Resource Centre, for providing the clone in any publication arising out of using this resource. Clones (e.g. 202-P6) should be cited as: "B202p06 from the Fugu BAC library (Dr. G. Elgar, UK HGMP Resource Centre)".

Version 1.0 9th January 2002