Clones are streaked onto LB agar containing kanamycin (25µg/ml).

To use: restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.

Single colonies are then ready for DNA isolation, if required, using the accompanying protocol.

Clones can be grown in LB broth + kanamycin, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.

Vector details

Vector: Lawrist 4
Vector size: 8.2kb
Antibiotic resistance: kanamycin
Inserts: Partial Sau3A1 genomic digest cloned into BamHI site.
Excise inserts using: not possible
Average insert size: 35-45kb

Multiple Cloning Site for Lawrist 4 vector

H = HindIII
B = BamHI

Further details are available from http://www-bio.llnl.gov/genome/html/diff.html

Subsequent publication:

Please acknowledge the originator of the library, Elgar and Nizetic, and Geneservice for providing the library in any publication arising out of using this resource. An example of how a clone (E.g. 171-m19) should be described in a publication is: 171m19 from the Elgar Fugu library.

Version 1.1 20th March 2001