All publications and database entries for this Drosophila cosmid library must be in the 96-well format. Below is an example of a Despatch Note printout indicating the correct ID to use:

Drosophila cosmid clones (in E.coli)

Clones are streaked onto LB agar containing kanamycin (25 µg/ml).

To use: re-streak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.

Single colonies are then ready for DNA isolation, using standard protocols, if required.

Clones can be grown in LB broth + kanamycin, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.

The vector used is Lorist 6 (Gibson. TJ. et al [1987] Gene 53: 283-286).

Version 2.2 12th August 2002