Fugu BAC Library
The Fugu rubripes BAC library, cloned into pBeloBACII
(
http://informa.bio.caltech.edu/protocols/BAC_lib_construction.html),
was constructed at Incyte using Fugu genomic DNA supplied by Dr Greg Elgar
(School of Biological Sciences,
Queen Mary, University of London,
Mile End Road, London, E1 4NS
).
The library contains 42,624 clones arrayed in 111 (384-well) plates. The
library
plates are numbered from 176-286.
Over half (23,453) of these clones have been HindIII fingerprinted
and
have an average insert size of 80kb, extrapolating to a nine-fold coverage
of
the genome. Fingerprint data is available from the
Fugu website
This library - along with the cosmid library also available through Geneservice Ltd
- underpins and is being integrated into the Fugu genome draft assembly. The
data will be linked together to form the long-range physical map of the Fugu
genome.
All the data linking Fugu draft assembly to BAC and cosmid clones is available from the Fugu website, allowing selection of genomic clones from any given region of
the Fugu
genome for further investigation or sequence finishing.
Individual clones from this library are now available.
Product Data Sheets
(technical information as supplied with order)
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