NIH Drosophila cDNA Library

This library was constructed by Brian Oliver at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) which is part of the National Institutes of Health (NIH) at Bethesda, MD, USA. Poly (A) + RNA was prepared from dissected testes from 1-5 day old y w [67cl] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.

The Resource Centre has approximately 3930 clones available from plates (labelled bs) numbered 3- 24, 26- 37, 40- 42, 44- 46 and 49. All plates except bs49 are available on one double-spotted high density filter for screening by hybridisation.