pTLJ03 6101 bp. Features: 1101-1958 ampR, 4656-5735 lacI, 2719-~2100 pBR322 ori, TSS (T7) 38, 21-37 T7 promoter, 94-101 RBS, 874-894 3RT1 recombinational tag, 841-861 5RT1 recombinational tag,823-840 6xHis tag, 109-780 gst tag, 790-804 enterokinase recognition site. 1 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa 61 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gtcccctata 121 ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt 181 gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa 241 aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa 301 ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt 361 tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac 421 ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc 481 aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat 541 ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac 601 atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa 661 gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag 721 ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatcggatct ggttccgcgt 781 ccatggtcgg acgatgacga taaggatcat atgtcgtact accatcacca tcaccatcac 841 ttgactgtat cgccggaatt cgggcccgga tccctgcagc caagctaatt ccggcttgaa 901 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 961 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1021 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1081 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1141 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1201 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1261 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 1321 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 1381 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 1441 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 1501 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 1561 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 1621 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 1681 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 1741 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 1801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 1861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 2281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 2341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 2401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 2461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 2521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 2581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 2641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 2701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 2761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 2821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 2881 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 2941 gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 3001 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg 3061 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 3121 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 3181 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc 3241 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata 3301 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg 3361 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg 3421 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta 3481 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca 3541 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg 3601 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt 3661 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt 3721 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac 3781 gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc 3841 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca 3901 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg 3961 aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg 4021 cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc 4081 tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg 4141 taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca 4201 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga 4261 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca 4321 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg 4381 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc 4441 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga 4501 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc 4561 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat 4621 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 4681 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 4741 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc 4801 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa 4861 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat 4921 cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg 4981 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc 5041 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc 5101 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag 5161 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc 5221 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg 5281 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca 5341 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg 5401 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg 5461 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg 5521 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc 5581 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc 5641 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca 5701 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct 5761 tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc 5821 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc 5881 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc 5941 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg 6001 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc 6061 gccggtgatg ccggccacga tgcgtccggc gtagaggatc g