C. jejuni ORF Library

Please note that the clone(s) being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.

THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER ). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.

WHAT TO DO WITH YOUR CLONES WHEN YOU RECEIVE THEM

Plates- contain LB broth with 8% glycerol and ampicillin. These are sent on Dry Ice. These should be stored at -70°C

Individual clones pools have been streaked onto LB agar containing ampycillin (100µg/ml). Please store them at 4°C (not in a freezer). As soon as possible, transfer the culture to LB broth containing ampicilllin + 8% glycerol and incubate overnight at 37°C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37°C for subsequent freezing at -70°C.

These clones are for research purposes only.

Genotype:

SURE strain: e14- (McrA-) D(mcrCB-hsdSMR-mrr)171 endA1 supE44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 (Kan^r) uvrC [F' proAB lacI^qZDM15 Tn10 (Tet^r)] (Stratagene)

Databases:

Please click on Cjejuni.xls to view database.

Vector information:

Please click on vector map to view vector map and vector sequence to view vector sequence.

Acknowledgements and Subsequent Publication:

We are grateful to Russell Finley & Jodi Parrish (Wayne State University School of Medicine) for the gift of this library and associated database
Please acknowledge the Wayne State University School of Medicine and Geneservice Ltd in any resulting publications.
Please also cite the following paper:
Parrish J, Limjindaporn T, Hines JA, Liu J, Liu G, and Finley RL, Jr. High-throughput cloning of Campylobacter jejuni ORFs by in vivo recombination in Escherichia coli, J. Proteome Research, May-June 2004; 3(3): 582-586.