Sequence Verified UK Chicken Consortium EST Clones

Geneservice will end sequence your clones before they are sent to you - you only pay for the clones that BLAST match with the id you requested. This risk free option saves you time when you get your clone and removes the chance of receiving one of the wrong clones that are inherent in all copies of large libraries.

Place your order conventionally but follow the Sequence Verified clones link Geneservice will extract DNA, end sequence your requested clone(s) and BLAST the output Successfully verified clones (>95% identity after quality clipping) will be shipped to you and we will include a BLAST report in the packet Customers will be notified in their delivery about the clones that fail verification, no charge will be made for failed clones. In the unlikely event that all clones in the order fail verification the order will be completely cancelled at no charge and customers will be contacted by email.

UK Chicken Consortium EST Clones

This project to produce a large collection of EST clones from the chicken was funded by the BBSRC and was directed by colleagues at the Universities of Nottingham, Dundee and UMIST. Sequencing was by Incyte Genomics Inc. All tissues were taken from the White Leghorn (Layer) breed (Gallus domesticus). The Adult Cerebrum, Adult Cerebellum and Adult Brain Other Parts tissues were from the White Leghorn IAH strain of RPL Line 15I.

All embryonic tissues were from the White Leghorn 'Hisex' breed. The remaining adult tissues (except muscle, chondrocyte, ovary, pancreas and adipose) originate from White Leghorn Line 15 hens.

Note: IAH = Institute of Animal Health, RPL = Regional Poultry Laboratory, East Lansing, Michigan. For more information please see the Chicken EST Repository Homepage (http://www.chick.umist.ac.uk/).

21 tissues have been sequenced, with each tissue having multiple cDNA libraries produced from different normalisation stringencies. The majority of the clones are in pBluescript II KS+ (Stratagene) in DH10B or DH10B-T1 resistant bacteria. EST sequences have been produced from the 5’ end of each clone.

Clones are currently supplied individually, with no limits on the numbers that may be ordered. For supply of clones in other formats (e.g. whole plates) please contact BioHelp.