Mouse cDNA NIA 15K Set
This mouse cDNA clone set was prepared by Dr Minoru Ko and colleagues
(Tanaka
et al., PNAS 97, 9127-9132) at the National
Institute on Aging which is one of the 25 institutes and
centres of the National Institutes
of Health.
Full details of this clone set can be obtained from http://lgsun.grc.nia.nih.gov/cDNA/15k.html.
The clone set has now been distributed widely and is currently
in use by a number of microarray programmes in many countries.
The set has the following characteristics:
- ~ 15,000 'unique' cDNA clones were rearrayed from 52,374
ESTs from pre- and periimplantation embryos, E12.5 female
gonad/mesonephros, and newborn ovary.
- Up to 50% are derived from novel genes.
- ~ 1.5 kb average insert size.
- Clones were sequenced from 5' and 3' termini to obtain
longer reads and verify sequence. Sequence information is
available from their web site (http://lgsun.grc.nia.nih.gov/cDNA/15k.html).
- 95% of the wells produce PCR products with 94% of the
products comprising a single amplification band.
This 15K mouse cDNA clone set is provided by Geneservice
as purified PCR products in 96-well plates. Each well contains
10µl of purified PCR product, at an average concentration
of 0.1mg/ml (as determined by A260 absorbance).
The PCR products are available for the whole clone set or
as part sets (5K) as follows:
NIA complete PCR set (159 plates numbered H3001-H3159)
NIA H3001-H3053 (Part 1, 53 plates numbered H3001-H3053)
NIA H3054-H3106 (Part 2, 53 plates numbered H3054-H3106)
NIA H3107-H3159 (Part 3, 53 plates numbered H3107-H3159)
To see agarose gel images of the PCR products from each of
the 159 plates please click here.
Individual bacterial clones are also available for this clone
set
|
